Publications – Kamat Lab

A complete list of Neha Kamat’s publications can be found at Google Scholar.

DNA-Directed Assembly of Multivalent Lipid Nanoparticles for Targeted T Cell Gene Delivery

Kelly, M. D., Vu, T. Q., Iyer, A. U., Luo, Y., Linderman, A. P., Cline, L., Sanchez, C., Kamat N. P.*

Journal of Controlled Release 2026, Volume 394, 114885

Controlled Assembly of Vesicle-Based Superstructures Using Megamolecules

Vu, T. Q., Sridhar, S., Shekour, B. G., Iyer, A., Cline, L., Kimmel, B. R., Modica J. A., Mocan, L., Mrksich, M.*, Kamat, N. P.*

ACS Applied Materials & Interfaces 2026, 18, 3, 4937–4951

Assessing protein-lipid interactions with a low-cost, accessible centrifugation assay

Villaseñor C. G., Karagiaridi, A., Dimitrova V. S., Buyco, D. G., Candal, I., Smirnova, A., Pinkett, H. W., Kamat, N. P.*

Biophysical Reports 2025, Volume 5, Issue 3, 100224

Engineered Membrane Vesicle Production via oprF or oprI Deletion Has Distinct Phenotypic Effects in Pseudomonas putida

Wilkes, R. A., Miller, T. E., Waldbauer, J., Zhou, N., Zhang, L., DiBiase, B., Kamat, N. P., Aristilde, L., Beckham, G. T., Werner, A. Z.*

ACS Synthetic Biology 2025, 14, 7, 2739–2752

Cell-free expression of Nipah virus transmembrane proteins for proteoliposome vaccine design

Hu, V. T., Ezzatpour, S., Selivanovitch, E. T., Carter, J., Sahler, J., Adeleke, R. A., August, A., Aguilar-Carreno, H.*, Daniel, S.*, Kamat, N. P.*

ACS Nano 2025, 19(23), 21290-21306

Melting Point Matters: Designing Lipid Nanocarriers for Improved T Cell Activation

Fedosejevs, C. S., Cline, L., Kamat, N. P.

Faraday Discussions 2025, 259, 129-148

Remodeling of lipid-foam prototissues by network-wide tension fluctuations induced by active particles

Gu, A. A., Can Uçar, M., Tran, P., Prindle, A., Kamat, N. P. & Steinkühler, J. *

Nature Communications 2025, 16(1), 2026

Probing the force-from-lipid mechanism with synthetic polymers

Jacobs, M. L., Steinkühler, J., Lemley, A., Larmore, M. J., Ng, L. C. T., Cologna, S. M., DeCaen*, P. G., Kamat, N. P.*

eLife 2025

A synthetic method to assay polycystin channel biophysics

Larmore, M., Palomero, O. E., Kamat, N. P., DeCaen, P. G.*

eLife reviewed preprint, 2024

Enhancing extracellular vesicle cargo loading and functional delivery by engineering protein-lipid interactions

Peruzzi, J. A., Gunnels, T. F., Edelstein, H. I., Lu, P., Baker, D., Leonard, J. N.*, & Kamat, N. P.*

Nature Communications, 2024, 15(1), 5618

Deconstructing synthetic biology across scales: a conceptual approach for training synthetic biologists

Karim, A. S.*, Brown, D. M., Archuleta, C. M., Grannan, S., Aristilde, L., Goyal, Y., Leonard, J. N., Mangan, N. M., Prindle, A., Rocklin, G. J., Tyo, K. J., Zoloth, L., Jewett, M. C., Calkins, S., Kamat, N. P., Tullman-Ercek, D., Lucks, J. B.*

Nature Communications, 2024, 15(1), 5425

A microfluidic platform for the synthesis of polymer and polymer-protein-based protocells

JO’Callaghan, J. A., Kamat, N. P., Vargo, K. B., Chattaraj, R., Lee, D.*, Hammer, D. A.*

The European Physical Journal E, 2024, 47(6), 37

Hydrophobic mismatch drives self-organization of designer proteins into synthetic membranes

Peruzzi, J. A., Steinkühler, J., Vu, T. Q., Gunnels, T. F., Hu, V. T., Lu, P., Baker, D., & Kamat, N. P.*

Nature Communications, 2024, 15(1), 3162

Progress in Engineering Synthetic Cells and Cell-Free Systems

Dogterom, M., Kamat, N. P., Jewett, M. C., and Adamala, K. P.*

ACS Synthetic Biology, 2024, 13(3), 695-696

Improving cell-free expression of membrane proteins by tuning ribosome co-translational membrane association and nascent chain aggregation

Steinküher, J.*, Peruzzi, J. A., Krüger, A, Jacobs, M. L., Jewett, M. C., Kamat, N.P.*

ACS Synthetic Biology, 2023, 13(1), 129-140

Engineering transmembrane signal transduction in synthetic membranes using two-component systems

Peruzzi, J. A., Galvez, N. R., Kamat, N. P.*

PNAS, 2023, 120 (19) e2218610120

Rapid Generation of Therapeutic Nanoparticles Using Cell-Free Expression Systems

Peruzzi, J. A., Vu, T. Q., Gunnels, T. F., Kamat, N. P.*

Small Methods, 2023, 7(12), 2201718

Tuning Targeted Liposome Avidity to Cells via Lipid Phase Separation

Vu, T. Q., Sant'Anna, L. E., Kamat, N. P.*

Biomacromolecules, 2023, 24, 4, 1574–1584

Engineered membrane receptors with customizable input and output functions

Peruzzi, J. A., Vu, T. Q., Kamat, N. P.*

Trends in Biotechnology, 2023

Cell-free protein synthesis systems for vaccine design and production

Hu, V. T., Kamat, N. P.*

Current Opinion in Biotechnology, 2023, 79, 102888

Robust and tunable performance of a cell-free biosensor encapsulated in lipid vesicles

Boyd, M. A., Thavarajah, W., Lucks, J. B.*, Kamat, N. P.*

Science Advances, 2023, 9, 1, eadd6605

Enzymatically-active bacterial microcompartments follow substrate gradients and are protected from aggregation in a cell-free system

Steinkühler, J.*, Abrahamson, C. H., Agudo-Canalejo, J., Golestanian, R., Tullman-Ercek, D.*, Kamat, N. P.*

bioRxiv 2022

PEO-b-PBD Diblock Copolymers Induce Packing Defects in Lipid/Hybrid Membranes and Improve Insertion Rates of Natively Folded Peptides

Steinkühler, J., Jacobs, M. L., Boyd, M. A., Villaseñor, C. G., Loverde, S. M., Kamat, N. P.*

Biomacromolecules, 2022, 23, 11, 4756-4765

Development of a Low-Cost, Easy-to-Adopt Diversity, Equity, and Inclusion Program During Crisis

Ankeny, C. J., Dunton, C., Virk, R., Scott, E., Kamat, N. P.

Biomedical Engineering Education, 2022, 1-7

Elucidating design principles for engineering cell-derived vesicles to inhibit SARS-CoV-2 infection

Gunnels, T.F., Stranford, D.M., Mitrut, R.E., Kamat, N.P.*, Leonard, J. N.*

Small 2022, 18, 19, 2200125

Lipid phase separation in vesicles enhances TRAIL-mediated cytotoxicity

Vu, T. Q., Peruzzi, J. A., Sant'Anna, L. E., Roth, E. W., Kamat, N.P.*

Nano Letters 2022, 22, 7, 2627-2634

Cell-Free Membrane Protein Expression into Hybrid Lipid/Polymer Vesicles

Jacobs, M. L. and Kamat, N. P.*

Cell-Free Gene Expression. Methods in Molecular Biology, 2022, 2433

Vesicle-based sensors for extracellular potassium detection

Boyd, M.A., Davis, A.M., Chambers, N.R., Tran, P., Prindle, A., Kamat, N.P. *

Cellular and Molecular Bioengineering, 2021, 14, 459-469

EPA and DHA differentially modulate membrane elasticity in the presence of cholesterol

Jacobs, M.L., Faizi, H.A., Peruzzi, J. A., Vlahovska, P.M., Kamat, N.P. *

Biophysical Journal, 2021, 120, 11, 2317-2329

Bioinspired Macromolecular Materials

Webber, M.J. , Kamat, N.P. , Messersmith, P.B. , Lecommandoux, S.

Biomacromolecules, 2021, 22, 1, 1-3

Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

Hershewe, J., Warfel, K., Iyer, S., Peruzzi, J., Sullivan, C.J., Roth, E.W., DeLisa, M.P., Kamat, N.P., Jewett, M.C.

Nature Communications, 2021, 12, 2363

Cell-Free Synthesis of a Transmembrane Mechanosensitive Channel Protein into a Hybrid-Supported Lipid Bilayer

Manzer, Z., Ghosh, S., Jacobs, M.L., Krishnan, S., Zipfel, W.R., Piñeros, M., Kamat, N. P. *, Daniel, S. *

Applied Bio Materials, 2021, 4, 3101-3112

Designing artificial cells towards a new generation of biosensors

Boyd, M. A and Kamat, N. P.*

Trends in Biotechnology, 2021, 39, 9, 927-939

Energy Dissipation at Interfaces Drives Multicompartment Remodeling

Steinkühler, J., and Kamat, N. P.*

Chem, 2020, 6, 1160-1171

Barcoding biological reactions with DNA-functionalized vesicles

Peruzzi, J. A., Jacobs, M. L., Vu, T. Q. and Kamat, N. P.*

Angew Chem Int Ed, 2019, 58, 18683-18690

Controlling secretion in artificial cells with a membrane AND gate

Hilburger, C. E., Jacobs, M. L., Lewis, K. R., Peruzzi, J. A. and Kamat, N. P.*

ACS Synthetic Biology, 2019, 8, 1224-1230

Diblock copolymers enhance folding of a mechanosensitive membrane protein during cell-free expression

Jacobs, M. L., Boyd, M. A., And Kamat, N. P.*

Proc. Natl. Acad. Sci. U.S.A., 2019, 116, 4031-4036

Visualizing tension and growth in model membranes using optical dyes

Boyd, M. A., And Kamat, N. P.*

Biophys J 2018, 115, 1307-1315

Copying of mixed sequence RNA templates inside model protocells

O’flaherty, D. K.*; Kamat, N. P.*^; Mirza, F. N.; Li, L.; Prywes, N.; Szostak, J. W.

JACS 2018, 140, 5171-517
*authors contributed equally; ^corresponding authors

Fatty Acid/Phospholipid Blended Membranes: A Potential Intermediate State in Protocellular Evolution

Jin, L.*; Kamat, N. P.*; Jena, S.; Szostak, J. W.

Small 2018, 14, 1704077
*authors contributed equally

N-Carboxyanhydride-Mediated Fatty Acylation of Amino Acids and Peptides for Functionalization of Protocell Membranes

Izgu, E. C.; Björkbom, A.; Kamat, N. P.; Lelyveld, V. S.; Zhang, W.; Jia, T. Z.; Szostak, J. W.

Journal of the American Chemical Society 2016, 138, 16669-16676

Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides

Kamat, N. P.; Tobe, S.; Hill, I. T.; Szostak, J. W.

Angewandte Chemie 2015, 54, 11735-11739

Construction of a Liposome Dialyzer for the Preparation of High-Value, Small-Volume Liposome Formulations

Adamala, K.; Engelhart, A. E.; Kamat, N. P.; Jin, L.; Szostak, J. W.

Nature Protocols 2015, 10, 927-38

Modular Synthesis of Amphiphilic Janus Glycodendrimers and Their Self-Assembly into Glycodendrimersomes and Other Complex Architectures with Bioactivity to Biomedically Relevant Lectins

Percec, V.; Leowanawat, P.; Sun, H. J.; Kulikov, O.; Nusbaum, C. D.; Tran, T. M.; Bertin, A.; Wilson, D. A.; Peterca, M.; Zhang, S.; Kamat, N. P.; Vargo, K.; Moock, D.; Johnston, E. D.; Hammer, D. A.; Pochan, D. J.; Chen, Y.; Chabre, Y. M.; Shiao, T. C.; Bergeron-Brlek, M.; Andre, S.; Roy, R.; Gabius, H. J .; Heiney, P. A.

Nature Protocols 2015, 10, 927-38